Lab Notes

Welcome to the new ATCC blog!

Welcome to the new ATCC blog! We have created this forum to provide a fun and educational way for Microbiologists and Cell Biologists to learn more about the natural world and to stay connected with new and exciting topics, techniques, and best practices in science.

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Propagation methods for anaerobes

By Allison FaustNancy Krueger

For anaerobic cultures, the exclusion of oxygen from the environment is essential for growth. Presently, there are a number of methods that can be used to ensure an oxygen-free environment. Here, we will discuss several common methods for the cultivation of anaerobic bacteria.

The basics of lyophilization

By Carol Horton

Lyophilization, or the process of freeze-drying, is a valuable method for preserving materials for longer-term storage and stability. Similar to cryopreservation, when we talk about lyophilization we focus on the management of water during the preservation process. However, during lyophilization, water is removed through a process called sublimation to yield a stable, dehydrated material with a relatively indefinite shelf-life.

The ideal characteristics of an in vitro whole-cell model include equivalence to in vivo physiology, genotypic and karyotypic stability, high proliferative capability, and the ability to be used at high passage. Does such a cell type exist? In this blog entry, I will discuss human telomerase (hTERT)-immortalized cells (ICs), which possess all of these qualities.

The basics of cryopreservation

By Carol Horton

Preservation becomes essential in the routine maintenance of cultures, allowing us to minimize passage in vitro and preserving valuable stocks used or developed in research. The most common methods of preservation include cryopreservation, which is the process of freezing cultures, and lyophilization, which is the process of freeze-drying material for longer-term storage and stability. Central to both techniques is the management of water during the preservation process. Here, we will discuss the basics of cryopreservation.

The Rise of Cell Authentication

By Maryellen de Mars

The issue of misidentified cell lines is nothing new to the scientific community, but recent attention toward addressing this persistent challenge seems to have increased. However, there has been considerable advances in rectifying the problem of irreproducibility in science and promoting the need for standards in addressing this issue.