Welcome to the new ATCC blog!

Welcome to the new ATCC blog! We have created this forum to provide a fun and educational way for Microbiologists and Cell Biologists to learn more about the natural world and to stay connected with new and exciting topics, techniques, and best practices in science.

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Long-term subculturing of a microbial strain poses both a risk for cross-contamination and places selective pressure on the strain’s traits, which may lead to detrimental effects such as genetic drift and phenotypic variation. Here, we will discuss the importance of standards in microbial research and assay development.

How to minimize oxygen toxicity when propagating anaerobes

By Nancy KruegerAllison Faust

Oxygen can be quite harmful and even toxic to all cells, resulting in the oxidation of membrane lipids, inactivation of enzymes, or genetic damage. Here, we discuss several defenses that bacteria have acquired to protect themselves from oxygen toxicity.

Choosing the best media for your anaerobe

By Allison FaustNancy Krueger

Understanding your anaerobe’s nutritional requirements is essential for selecting the correct media. Presently, there are a number of broth and agar media that can be freshly prepared or purchased from commercial suppliers to support the growth of your cultures. Here, we will provide an overview of these types of media as well as the importance of using indicators and reducing agents.

Propagation methods for anaerobes

By Allison FaustNancy Krueger

For anaerobic cultures, the exclusion of oxygen from the environment is essential for growth. Presently, there are a number of methods that can be used to ensure an oxygen-free environment. Here, we will discuss several common methods for the cultivation of anaerobic bacteria.

The basics of lyophilization

By Carol Horton

Lyophilization, or the process of freeze-drying, is a valuable method for preserving materials for longer-term storage and stability. Similar to cryopreservation, when we talk about lyophilization we focus on the management of water during the preservation process. However, during lyophilization, water is removed through a process called sublimation to yield a stable, dehydrated material with a relatively indefinite shelf-life.